Immobilization of beneficial microbe Methylobacterium aminovorans in electrospun nanofibre as potential seed coatings for improving germination and growth of groundnut Arachis hypogaea

Seed inoculation with microbial cells is one of the potential invigouration techniques for enhancing emergence and growth plants. Herein, we approached a new localized delivery beneficial (Methylobacterium) by invigorating seeds electrospun Polyvinyl alcohol (PVA) nanofibre containing cells. Methylobacterium promoting bacteria that has recently drawn attention in agriculture, particularly drought management. PVA was used this research because its electrospinnability biodegradability. Encapsulation study shows effective immobilization (Methylorubrum aminovorans) nanofibre. SEM TEM characterization further confirmed entrapment The plating enumeration reveals 6.6 × 105 CFU g−1 to initial loading population 1 108 CFU. Viability encapsulated bacterial under ambient environment found 1.85 g−1, 2.2 104 1.2 on 10, 20 30 days after storage, respectively. In vitro bio-efficacy exhibits coated nanofibres M. aminovorans recorded higher germination, root & shoot length, seedling vigor, drymatter production, plant biomass, volume, nodule numbers fresh weight nodules. concludes could be immobilized extended shelf-life as an seed coating delivery.